Merck
CN

G5537

Sigma-Aldrich

甘油醛-3-磷酸脱氢酶 来源于面包酵母(酿酒酵母

lyophilized powder, 70-140 units/mg protein

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别名:
D-甘油醛-3-磷酸:NAD+ 氧化还原酶(磷酸化), GAPDH
CAS号:
EC 号:
MDL编号:

形式

lyophilized powder

质量水平

比活

70-140 units/mg protein

组成

Protein, 30-70% biuret

异质活性

3-Phosphoglyceric phosphokinase ≤0.05%

储存温度

−20°C

生化/生理作用

甘油醛-3-磷酸脱氢酶催化3-醛甘油醛向1,3-双磷酸甘油酯的转化,作为糖酵解的一部分。 它也被证明在凋亡启动、转录激活和内质网向高尔基囊泡的穿梭中起作用 <<<,New> >>。

单位定义

在25°C的pH值为7.6的情况下,在具有3-磷酸甘油磷酸激酶的偶联系统中,每单位将每分钟将1.0μmol的3-磷酸甘油酯还原为D-甘油醛3-磷酸。

外形

用海藻糖、柠檬酸盐和DTT稳定的冻干的无硫酸盐粉末。 对于需要低硫酸盐的系统很有用。

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Lei Tong et al.
The Journal of biological chemistry, 284(17), 11256-11266 (2009-03-03)
Although the classical redox functions of co-enzyme NAD(+) are firmly established in metabolism, there are numerous enzymes that catalyze cleavage of NAD(+) to yield free ADP-ribose (ADPr) or related metabolites, whose functions remain largely unknown. Here we show that the
A Tarze et al.
Oncogene, 26(18), 2606-2620 (2006-10-31)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Here, we report that the protein accumulates in mitochondria during apoptosis, and induces the pro-apoptotic mitochondrial membrane permeabilization, a decisive event of
Soya S Sam et al.
Pathology, research and practice, 208(12), 705-707 (2012-10-13)
Isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue remains a laborious task for clinical laboratories and researchers who need to screen several samples for genetic variants. The objective of this study was to evaluate DNA isolation methods from FFPE tissues
Qiao Liu et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 8), 978-980 (2012-08-08)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an important enzyme in the glycolytic pathway. In addition to its conventional metabolic role, GAPDH has been identified to possess diverse cellular functions. In this study, glyceraldehyde-3-phosphate dehydrogenase 3, the third isoform of GAPDH from Saccharomyces
Shunqing Liang et al.
Veterinary immunology and immunopathology, 150(3-4), 198-205 (2012-10-23)
Edwardsiella tarda is a Gram-negative pathogen which causes systemic infection in turbot. The increasing frequency of edwardsiellosis in turbot farming has stressed the need to understand the immune responses of fish, for the further development of prevention and control strategies.

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