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Merck
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G5885

葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌

lyophilized powder, >= 550 units/mg protein (biuret)

别名:

G-6-P-DH

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化学文摘社编号:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
MFCD00081656
EC 号:
1.1.1.49 (BRENDA, IUBMB)

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产品名称

葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌, lyophilized powder, >= 550 units/mg protein (biuret)

biological source

bacterial (Leuconostoc mesenteroides)

type

Type XXIV

form

lyophilized powder

specific activity

>= 550 units/mg protein (biuret)

mol wt

128 kDa

composition

Protein, 15-40% biuret

application(s)

agriculture

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%

storage temp.

2-8°C

Quality Level

300

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相关类别

Application

来自肠膜明串珠菌的葡萄糖-6-磷酸脱氢酶已联用己糖激酶进行小鼠肝样品中的葡萄糖测定。

Biochem/physiol Actions

葡萄糖-6-磷酸脱氢酶(G-6-PDH)可使用烟酰胺腺嘌呤二核苷酸磷酸(NADP+)或NAD+作为辅酶，在细菌代谢中具有重要作用。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。

General description

葡萄糖-6-磷酸脱氢酶(G-6-PDH)由His-Asp催化二联体构成，并以同二聚体形式存在。结构上，G-6-PDH在辅酶结合结构域内包含罗斯曼折叠构成的二核苷酸结合区域。还具有一个较大的β + α结构域，在374位有一个独特的天冬氨酸残基。

Other Notes

一个单元在NAD 存在下，在pH 7.8，30℃ 下，每分钟将 1.0 μmole的D-葡萄糖-6-磷酸氧化成 6-磷酸-D-葡萄糖酸盐。

Physical form

含有Ficoll和Tris 缓冲盐的冻干粉

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000476088
0000476088
0000402989
0000402990
0000402988

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?

    The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).

  6. Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc  mesenteroides, be stored?

    Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.

  7. What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

  8. What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    Product No. G8529 is a recombinant protein from Leuconostoc  mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M S Cosgrove et al.
Biochemistry, 39(49), 15002-15011 (2000-12-07)
The role of Asp-177 in the His-Asp catalytic dyad of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides has been investigated by a structural and functional characterization of the D177N mutant enzyme. Its three-dimensional structure has been determined by X-ray cryocrystallography in
Hana A Dibe et al.
Physiological reports, 8(3), e14370-e14370 (2020-02-16)
The liver is the primary metabolic organ involved in the endogenous production of glucose through glycogenolysis and gluconeogenesis. Hepatic glucose production (HGP) is increased via neural-hormonal mechanisms such as increases in catecholamines. To date, the effects of prior exercise training
Enzyme-stabilizing activity of seed trypsin inhibitors during desiccation
Ji-Ming Lam, Keng-Hock Pwee, Wendell Q. Sun, Yii-Leng Chua, Xing-Jun Wang
Plant Science, 142, 209-218 (1999)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
V Vought et al.
Biochemistry, 39(49), 15012-15021 (2000-12-07)
The roles of particular amino acids in substrate and coenzyme binding and catalysis of glucose-6-phosphate dehydrogenase of Leuconostoc mesenteroides have been investigated by site-directed mutagenesis, kinetic analysis, and determination of binding constants. The enzyme from this species has functional dual
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实验方案

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

相关内容

Product Information Sheet - G5885

Product Information Sheet

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