一般描述
谷氨酸是非必需氨基酸。是主要的兴奋性神经递质。在重大创伤、重大手术、败血症和骨髓移植中,谷氨酸起必需氨基酸的作用。
应用
从链霉菌( Streptomyces )属提取的 L-谷氨酸氧化酶用于确定固定化谷氨酸氧化酶(GOx)的酶活性。也作为随机光学重建显微镜(STORM)成像缓冲液成分。
生化/生理作用
L-谷氨酸氧化酶催化 L-谷氨酸转化为 2-酮戊二酸。
谷氨酸合成或降解中的主要酶;将氨基转移至α-酮戊二酸形成 L-谷氨酸或脱酰胺基 L-谷氨酸。
其他说明
在 30°C、pH 7.4 条件下 1 个单位 α-酮戊二酸的生成速率为 1.0 μmol/min。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
II. Glutamine and glutamate
Tapiero H, et al.
Biomedicine and Pharmacotherapy, 56(9), 446-457 (2002)
Purification and properties of a new enzyme, L-glutamate oxidase, from Streptomyces sp X-119-6 grown on wheat bran.
H. Yoshino
Agricultural and Biological Chemistry, 74, 1323-1328 (1983)
Stefania Digiovanni et al.
Scientific reports, 10(1), 10135-10135 (2020-06-25)
Reactive Intermediate Deaminase (Rid) protein superfamily includes eight families among which the RidA is conserved in all domains of life. RidA proteins accelerate the deamination of the reactive 2-aminoacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes. 2AA
Jiro Arima et al.
Journal of biochemistry, 134(6), 805-812 (2004-02-11)
L-glutamate oxidase (LGOX) from Streptomyces sp. X-119-6 is a protein of 150 kDa that has hexamer structure alpha2beta2gamma2. The gene encoding LGOX was cloned and heterologously expressed in Escherichia coli. LGOX isolated from the E. coli transformant had the structure
Rasa Pauliukaite et al.
Analytical and bioanalytical chemistry, 386(2), 220-227 (2006-08-19)
An amperometric biosensor has been developed for measurement of Umami, or the taste based on the amount of L-glutamate, in tomato foods. The biosensor is based on an enzyme-mediator system in which L-glutamate oxidase is used for biochemical oxidation of
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