G6008
β 半乳糖苷酶 来源于大肠杆菌
Grade VI, lyophilized powder, ≥250 units/mg protein
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
产品名称
β 半乳糖苷酶 来源于大肠杆菌, Grade VI, lyophilized powder, ≥250 units/mg protein
biological source
Escherichia coli
type
Grade VI
form
lyophilized powder
specific activity
≥250 units/mg protein
mol wt
465 kDa
does not contain
BSA as extender
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
β-半乳糖苷酶与特异性识别目标分子的抗体偶联（酶联免疫检测或 EIA）。 β-半乳糖苷酶还被用作监测启动子基因表达水平的报告酶。
Biochem/physiol Actions
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
β-半乳糖苷酶将乳糖切割成单糖成分，即葡萄糖和半乳糖。它还在反馈环中催化葡萄糖的转糖基化，生成β-半乳糖苷酶的诱导物——异乳糖。
General description
β-半乳糖苷酶是由四个相同亚基组成的四聚体，每个亚基为135,000 Da。它是一种含巯基的酶，每个亚基含有19个半胱氨酸残基。
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
Other Notes
在 37 °C，pH 7.3 条件下，一个单位每分钟可将 1.0μmole 邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D-半乳糖。
Physical form
部分纯化；含有Tris缓冲盐、氯化镁、DL-二硫苏糖醇和 2-巯基乙醇
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
David P Klebl et al.
Frontiers in molecular biosciences, 9, 945772-945772 (2022-08-23)
Advances in single particle cryo-EM data collection and processing have seen a significant rise in its use. However, the influences of the environment generated through grid preparation, by for example interactions of proteins with the air-water interface are poorly understood
Orit Redy-Keisar et al.
Nature protocols, 9(1), 27-36 (2013-12-07)
This protocol describes the synthesis of modular turn-ON QCy7-based probes for the detection of biologically relevant analytes, such as hydrogen peroxide, ubiquitous sulfhydryl and β-galactosidase. The probes presented herein are prepared through a simple procedure that involves the preliminary alkylation
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
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