recombinant
expressed in E. coli
Quality Level
form
solution
specific activity
≥1400 unit/μg protein
mol wt
42 kDa
shipped in
dry ice
storage temp.
−70°C
General description
N-terminal GST-tagged 42 kDa full length protein
Biochem/physiol Actions
Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway.
Physical form
Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.
Other Notes
One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Dariusz Abramczyk et al.
Eukaryotic cell, 11(3), 334-342 (2012-01-03)
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and
Shivani Malik et al.
Nucleic acids research, 40(8), 3348-3363 (2011-12-27)
Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes
Aurélie Egert et al.
Plant & cell physiology, 53(5), 921-929 (2012-03-23)
Galactokinase (GALK, EC 2.7.1.6) is a cytosolic enzyme with a wide occurrence across the taxonomic kingdoms. It catalyzes the phosphorylation of α-d-galactose (Gal) to α-d-Gal-1-P. The cytotoxicity of free (unphosphorylated) Gal is well documented in plants and causes marked defects.
Erin M Green et al.
Molecular biology of the cell, 23(7), 1367-1375 (2012-02-11)
The genome is nonrandomly organized within the nucleus, but it remains unclear how gene position affects gene expression. Silenced genes have frequently been found associated with the nuclear periphery, and the environment at the periphery is believed to be refractory
Nils Janzen et al.
Archives of medical research, 42(7), 608-612 (2011-12-14)
Galactokinase (GALK) deficiency is an autosomal recessive disorder causing cataract formation that can be prevented or mitigated by early diagnosis and galactose-restricted diet. The aim of this retrospective study was to explore whether GALK-deficiency meets the criteria for neonatal mass
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G6048-200UG | 04061833638804 |
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