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G6392

L-谷氨酰胺

Name: <SC>L</SC>-谷氨酰胺
Brand: SIGMA

γ-irradiated, BioXtra, suitable for cell culture

别名:

(S)-2,5-二氨基-5-氧代戊酸, L-谷氨酸-5-酰胺, 左谷酰胺

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关于此项目

线性分子式:

H₂NCOCH₂CH₂CH(NH₂)CO₂H

化学文摘社编号:

56-85-9

分子量:

146.14

MDL number:

MFCD00008044

UNSPSC Code:

12352209

eCl@ss:

32160406

PubChem Substance ID:

24895254

EC Number:

200-292-1

NACRES:

NA.75

Beilstein/REAXYS Number:

1723797

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Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

powder

packaging

vial of 0.292 g

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to faint yellow

mp

185 °C (dec.) (lit.)

solubility

H₂O: 10 mL/vial, clear to slightly hazy

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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Application

L-谷氨酰胺已作为培养基补充剂用于：

耳蜗器官型培养
Iscove′s改良Dulbecco′s培养基中，以进行HeLa细胞培养
Dulbecco′s改良Eagle′s培养基中，以进行肝细胞原代培养

适用于细胞培养应用，尤其是无血清细胞培养。

Biochem/physiol Actions

谷氨酰胺通过细胞mTOR（雷帕霉素的哺乳动物靶标）信号转导介导蛋白质的周转。其还已报到可与细胞凋亡的抑制有关。

L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而，L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

L-谷氨酰胺是一种必需氨基酸，是培养基的重要成分，也是培养细胞的主要能源。最佳的细胞性能通常需要在使用前添加 L-谷氨酰胺的培养基。

Packaging

带橡胶塞的 50 mL 琥珀色血清瓶。

Preparation Note

用无菌水复溶至 10 mL 时，制备 200 mM 溶液。

Other Notes

二肽 L-Ala-LGln 经常用于现代无血清培养基配方中，因为它在体外提供了更稳定的谷氨酰胺形式。

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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全球贸易项目编号

货号	GTIN
G6392-1VL	04061833639122
G6392-10VL	04061833639115

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