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Merck
CN

G7396

β-葡萄糖醛酸酶 来源于大肠杆菌

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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关于此项目

化学文摘社编号:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
MFCD00199013
EC 号:
3.2.1.31 (BRENDA, IUBMB)
Specific activity:
1,000,000-5,000,000 units/g protein (30 min assay)

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type

Type IX-A

form

lyophilized powder

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

mol wt

290 kDa

composition

Protein, 30-70% biuret

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

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相关类别

Application

比较不同酶性能的新技术文章
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了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

Analysis Note

含有缓冲盐和稳定剂。

Other Notes

一个Sigma或改良Fishman单位是指在pH 6.8、温度37°C条件下（30分钟测定），每小时从苯酚葡萄糖醛酸中释放出1.0 μg酚酞所需的酶量。

pictograms

Health hazard
GHS08

signalword

Danger

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000533068
0000533071
0000456766
0000456766
0000533068

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Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

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