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Merck
CN

G7509

Sigma-Aldrich

GABase 来源于荧光假单胞菌

lyophilized powder, Protein ≥30 % by biuret

别名:

GABase酶, GABase酶,来自假单胞菌, 假单胞菌GABase酶

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关于此项目

MDL编号:
MFCD00131113
UNSPSC代码:
12352204
NACRES:
NA.32

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生物来源

Pseudomonas fluorescens

质量水平

200

方案

≥30  % (biuret)

表单

lyophilized powder

比活

≥0.5 units/mg protein

组成

Protein, ≥30% biuret

技术

inhibition assay: suitable

储存温度

−20°C

一般描述

研究领域：神经科学


GABase是一种市售酶混合物，由来自于荧光假单胞菌（Pseudomonas fluorescens）的γ-氨基丁酸氨基转移酶（GABGT）和琥珀酸半醛脱氢酶（SSDH）组成。

应用

来自荧光假单胞菌（Pseudomonas fluorescens）的GABase已被用于GABase测定，以测量GABA（γ-氨基丁酸）的细胞外和细胞内浓度。

生化/生理作用

GABase 催化中枢神经系统中的γ-氨基丁酸（GABA）转化为琥珀酸。它用于测定各种生物学样品中的GABA水平。对羟基苯甲醛（HBA）是潜在的GABase抑制剂。

外形

部分纯化的冻干粉末，含有缓冲盐和稳定剂

其他说明

一个酶活性单位是指在pH 8.6、25°C下，每分钟将1.0 μmole的γ-氨基丁酸（GABA）转化为琥珀酸半醛、再转化为琥珀酸，并且化学计量还原1.0 μmole的NADP+所需的酶量。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000458770
0000458770
0000436124
0000436124
0000412260

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?

    Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.

  6. What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?

    Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.

  7. Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?

    The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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