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Merck
CN

G8132

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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关于此项目

化学文摘社编号:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
MFCD00130629
EC 号:
3.2.1.31 (BRENDA, IUBMB)

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产品名称

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata), Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

biological source

limpet (Patella vulgata)

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

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相关类别

Analysis Note

含有被 0.1 M 磷酸盐抑制的硫酸酯酶活性。

Application

比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性的影响，导致 6-MAM 转化为吗啡
已证明β-L-II 型葡糖醛酸酶是尿液中药物葡糖醛酸苷水解的优越酶。
据报道，来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸

Biochem/physiol Actions

β-葡萄糖醛酸酶（β-GIc）是一种外切糖苷酶，催化复合碳水化合物的分解。在人体内可将结合胆红素转化为非结合形式，使胆红素适于重吸收。使用从 Patella vulgate 获得的 ß-葡糖苷酸酶将 3-β-D 葡糖苷酸转化为吗啡。

Other Notes

在37℃，pH 3.8（30分钟检测）条件下，一个Sigma或改良的Fishman单位可每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000489744
0000489744
0000457770
0000457770
0000457769

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Robert West et al.
Pain physician, 13(1), 71-78 (2010-02-02)
Physicians determine patient compliance with their medications by use of urine drug testing. It is known that measurement of benzodiazepines is limited by immunoassay specificity and cutoff limits and therefore does not offer physicians an accurate picture of their patients'
Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>
S.V. Marathe &amp; J.E. McEwen
Gene, 154, 105-107 (1994)
S Jain et al.
Nature structural biology, 3(4), 375-381 (1996-04-01)
The X-ray structure of the homotetrameric lysosomal acid hydrolase, human beta-glucuronidase (332,000 Mr), has been determined at 2.6 A resolution. The tetramer has approximate dihedral symmetry and each promoter consists of three structural domains with topologies similar to a jelly
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
J D McCarter et al.
Current opinion in structural biology, 4(6), 885-892 (1994-12-01)
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses
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实验方案

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使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

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