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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥20,000 units/mg protein
Recombinant:
expressed in E. coli overproducing strain
recombinant
expressed in E. coli overproducing strain
form
lyophilized powder
specific activity
≥20,000 units/mg protein
mol wt
290 kDa
composition
, ~25%
storage temp.
−20°C
Quality Level
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Analysis Note
含有缓冲盐和稳定剂。
Other Notes
一个Sigma或改良的Fishman单位将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0μg酚酞。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Enzymes
Hans Ulrich Bergmeyer
Methods of Analysis, 2, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
Amichay Meirovitz et al.
The FEBS journal, 280(10), 2307-2319 (2013-02-13)
Recent years have seen a growing body of evidence that enzymatic remodeling of heparan sulfate proteoglycans profoundly affects a variety of physiological and pathological processes, including inflammation, neovascularization, and tumor development. Heparanase is the sole mammalian endoglycosidase that cleaves heparan
Shu Feng et al.
Cancer research, 73(8), 2551-2562 (2013-02-27)
Prostate cancer is the most common visceral malignancy and the second leading cause of cancer deaths in US men. There is broad evidence that fibroblast growth factor (FGF) receptors are important in prostate cancer initiation and progression, but the contribution
Anjum Riaz et al.
The Journal of biological chemistry, 288(17), 12366-12375 (2013-03-19)
Heparanase functions as a heparan sulfate-degrading enzyme and as a ligand for an unidentified signaling receptor(s). Here, several reactions involved in the activation of the PI3K-AKT pathway by latent heparanase were characterized. Protein suppression using specific siRNAs revealed that heparanase-induced
商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
实验方案
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
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