G8511
β 半乳糖苷酶 来源于大肠杆菌
For use as a marker in SDS-PAGE
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
分子量
~116 kDa
包装
vial of 0.5 mg
储存温度
2-8°C
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一般描述
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
应用
来自 大肠杆菌 的β-半乳糖苷酶已被用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 的内标物。它还被应用于蛋白质梯形混合液中以优化微流体免疫印迹测定。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
其他说明
一个单位将在37°C 、 pH 7.3 下每分钟将 1.0μ摩尔邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D -半乳糖。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Scott W McPherson et al.
Journal of immunology (Baltimore, Md. : 1950), 190(10), 4971-4981 (2013-04-16)
Extrathymically derived regulatory T cells (iTregs) protect against autoimmunity to tissue-specific Ags. However, whether Ag-specific iTreg generation and function is limited to secondary lymphoid tissue or whether it can occur within the tissue-specific local environment of the cognate Ag remains
Staci Rakowiecki et al.
Development (Cambridge, England), 140(8), 1730-1739 (2013-03-15)
The morphogenetic program that shapes the three semicircular canals (SSCs) must be executed with extreme precision to satisfy their complex vestibular function. The SSCs emerge from epithelial outgrowths of the dorsal otocyst, the central regions of which fuse and resorb
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
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