G8511
β 半乳糖苷酶 来源于大肠杆菌
For use as a marker in SDS-PAGE
别名:
β-D-半乳糖苷半乳糖水解酶, 乳糖酶
mol wt
~116 kDa
packaging
vial of 0.5 mg
storage temp.
2-8°C
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General description
四聚体的分子量为465 kDa（每个亚基116.3 kDa）
Application
来自 大肠杆菌 的β-半乳糖苷酶已被用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 的内标物。它还被应用于蛋白质梯形混合液中以优化微流体免疫印迹测定。
Biochem/physiol Actions
β-半乳糖苷酶可将乳糖切割成单糖成分（葡萄糖和半乳糖）。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
Other Notes
一个单位将在37°C 、 pH 7.3 下每分钟将 1.0μ摩尔邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和 D -半乳糖。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
Javier A Menendez et al.
Cell cycle (Georgetown, Tex.), 12(4), 555-578 (2013-02-02)
Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of the nutrient-sensing mTOR gerogene. mTOR-driven aging can be triggered or accelerated by a decline or loss of responsiveness to activation of the energy-sensing protein AMPK, a critical
Hongmarn Park et al.
Nucleic acids research, 41(6), 3787-3804 (2013-02-09)
An artificial small RNA (afsRNA) scaffold was designed from an Escherichia coli sRNA, SibC. Using the lacZ reporter system, the gene silencing effects of afsRNAs were examined to explore the sRNA-mediated gene-silencing mechanisms in E. coli. Substitution of the original
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