G8885
β-葡萄糖醛酸酶 来源于罗曼蜗牛
Type H-3, aqueous solution, ≥90,000 units/mL
别名:
β-葡萄糖醛酸苷酶
产品名称
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-3, aqueous solution, ≥90,000 units/mL
type
Type H-3
form
aqueous solution
specific activity
≥90,000 units/mL
secondary activity
≤1,000 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Other Notes
在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
Physical form
在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。
Analysis Note
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
Application
β-葡萄糖醛酸苷酶可用于通过尿液技术进行的芳香化测定，适用于对芳香化酶抑制剂进行体内评估 。
来自勃艮第蜗牛的β-葡萄糖醛酸苷酶已被用于：
- 在尿液和豆浆样品中，将异黄酮结合物转化为苷元形式，从而用于高效液相色谱(HPLC)检测
- 用作原生质体溶液中的成分
- 用于从血浆和尿液样品中生成芳香族和酚类化合物，从而用于气相色谱-质谱(GC-MS)
- 水解粪便样品中的葡萄糖醛酸苷和硫酸盐共轭代谢物
Biochem/physiol Actions
β-葡萄糖醛酸酶（β-GIc）是一种外切糖苷酶，可催化复合碳水化合物分解。在人体内可将结合胆红素转化为非结合形式，使胆红素适于重吸收。
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
General description
来自勃艮第蜗牛的β-葡萄糖醛酸苷酶H-2型是一种源自罗马蜗牛的酶的粗溶液。许多衍生自软体动物的β-葡萄糖醛酸苷酶也具有硫酸酯酶活性。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
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商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
实验方案
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。
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