产品名称
Gentamicin-Glutamine solution, with 200 mM L-glutamine and 5 mg gentamicin per mL in tissue culture grade water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture
sterility
0.1 μm filtered
product line
BioReagent
form
liquid
potency
5-6 mg per mL
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria, mycoplasma
mode of action
protein synthesis | interferes
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
Chemical structure: aminoglycoside
Application
Gentamicn-Glutamine solution is used for selection of transformed cells that contain the aacA.aphD gene, and is recommended for use in cell culture applications at 10 mL/L.
Biochem/physiol Actions
Mode of Action: The product inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit. Glutamine functions as an essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeletons.
Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.
Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.
Other Notes
This solution is formulated to contain 200 mM L-glutamine and 5 mg/mL gentamicin in .9% sodium chloride
Disclaimer
This product is stable in solution at 37°C for 5 days. The recommended storage temperature is -20°C.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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Roger D Lawrie et al.
Pesticide biochemistry and physiology, 167, 104607-104607 (2020-06-13)
Human exposure to environmental chemicals both individually and in combination occurs frequently world-wide most often with unknown consequences. Use of molecular approaches to aide in the assessment of risk involved in chemical exposure is a growing field in toxicology. In
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Importance and uses of glutamine in hybridoma and mammalian cell culture
L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用
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