产品名称
鸟苷 5′--三磷酸盐-琼脂糖, aqueous glycerol suspension
SMILES string
[X]O[C@H]1[C@@H](O[C@@H]([C@H]1O[X])CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O)[n]2c3nc(nc(c3nc2)O)N
biological source
bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast
form
aqueous glycerol suspension
extent of labeling
1-5 μmol per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
ribose hydroxyls
matrix spacer
11 atoms (adipic acid dihydrazide)
storage temp.
−20°C
Quality Level
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相关类别
Application
鸟苷 5′-三磷酸-琼脂糖(5′-GTP 琼脂糖)是甘油水溶液悬浮液中的琼脂糖结合物,用于亲和层析和纯化鸟苷结合蛋白
Physical form
悬浮于含有0.25 M NaCl的50%甘油中
鸟苷5′-三磷酸-琼脂糖已被用于:
- 用于纯化易位刺激因子(translocation stimulating factor)的色谱柱
- 在GTP-琼脂糖拉下实验(pull down assay)中测量与GTP结合的RHEB(脑Ras同源蛋白,Ras homolog enriched in brain)的水平
- GTP结合测定中
- 消耗大鼠肝细胞溶质中的GTP结合蛋白
存储类别
10 - Combustible liquids
wgk
WGK 3
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The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich repeat kinase 2 (LRRK2), promoting self-association and cellular redistribution
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Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted
Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor
Bomsel M and Mostov KE
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The hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme in Trypanosoma cruzi is a rational target for the treatment of Chagas disease. To evaluate the T. cruzi HGPRT in detail, the HGPRT gene (hgprt) was cloned from a genomic library of T. cruzi DNA
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