包装
pkg of 10 ea
制造商/商品名称
Cytiva 11-0033-99
柱床体积
1 mL
色谱柱内径
15 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium)
工作范围
3-12(Ni2+-stripped medium.)
容量
~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)
适用性
suitable for bioprocess medium
一般描述
His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
特点和优势
- For fast and simple purification of histidine-tagged* proteins.
- Convenient, prepacked gravity-flow column.
- Allows direct purification of both clarified and unclarified cell lysates.
- High binding capacity; approximately 40 mg of pure protein per column.
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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