form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 11-0034-95
storage condition
(20% Ethanol)
parameter
2.5 mL/min flow rate (recommended), 20 mL/min max. flow rate (flow rate: 42 p), 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
highly cross-linked agarose
matrix active group
alkali-stabilized protein A-derived (E. coli)
avg. part. size
~85 μm
cleaning
3-13.7
working range
3-12
capacity
~35 mg binding capacity (huamn IgG / mL resin)
separation technique
affinity
storage temp.
2-8°C
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相关类别
General description
HiTrap® MabSelect™ SuRe™ columns are prepacked with MabSelect™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).
HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.
Features and Benefits
- Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.
Preparation Note
Analysis Note
Other Notes
Legal Information
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.
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