feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 500 g
manufacturer/tradename
Cytiva 17-0010-02
parameter
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
matrix
cross-linked dextran
particle size
40-120 μm (dry), 55-165 (wet)
working range
2-13
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General description
Sephadex® G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 700 molecular weight. Smallest Exlusion limit in the Sephadex® range.
Application
Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 700 molecular weight
- Efficient removal of contaminants such as salts, dyes, and radioactive labels
- Lowest exclusion limit (700 molecular weight) in the Sephadex® G-range
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
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实验方案
Column Packing and Preparation for Size Exclusion Chromatography
相关内容
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
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