form
resin (dry)
crosslinking
cross-linked
mol wt
protein Mr 800-5000 Da (fractionation range), protein Mr 8000 Da (exclusion limit)
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0033-01
storage condition
dry media
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
particle size
50-150 μm (dry), 85-255 μm (wet)
cleaning in place
2-13
operating pH
2-13
separation technique
size exclusion (SEC)
storage temp.
2-30°C
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General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).
Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Excellent recovery and minimum sample dilution
- Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
- BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
Preparation Note
Analysis Note
Legal Information
存储类别
13 - Non Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
This page shows how to prepare samples for purification with affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
相关内容
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
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