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Merck
CN

GE17-0041-01

葡聚糖凝胶 ® G-50

Superfine, Cytiva, pack of 100 g

别名:

SEC resin, buffer exchange resin, chromatography resin, cross-linked dextran, cytiva 17-0041-01, desalting matrix, filtration gel, size-exclusion resin

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化学文摘社编号:
9004-54-0
MDL编号:
MFCD23701222.obs1210407
UNSPSC代码:
41115722
NACRES:
NA.56

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表单

resin

交联

cross-linked

分子量

protein Mr 3-20 kDa (fractionation range)
protein Mr 30 kDa (exclusion limit)

特点

autoclavable In wet form (pH 7.0) at 120°C for 30 min

包装

pack of 100 g

制造商/商品名称

Cytiva

技术

liquid chromatography (LC): suitable

基质

cross-linked dextran

基质活性基团

dextran

溶胀

1 g swells to 12 mL

粒径

25-50 μm (dry)
51-106 μm (wet)

cleaning in place

2-13

工作范围

2-12

分离技术

size exclusion (SEC)

运输

ambient

储存温度

2-30°C

SMILES字符串

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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相关类别

一般描述

Sephadex® G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

特点和优势

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Small bead size for shorter diffusion distances
  • Smallest bead size in the Sephadex® G-50 range

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sephadex is a registered trademark of Cytiva

储存分类代码

13 - Non Combustible Solids

法规信息

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Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure

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