SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
form
resin
crosslinking
cross-linked
mol wt
protein Mr 3-20 kDa (fractionation range), protein Mr 30 kDa (exclusion limit), Mp 0.5-10 kDa (dextrans fractionation range)
feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 100 g
manufacturer/tradename
Cytiva
technique(s)
liquid chromatography (LC): suitable
matrix
cross-linked dextran
matrix active group
dextran
swelling
1 g swells to 14-15 mL
particle size
103-320 μm (wet), 50-150 μm (dry)
cleaning in place
2-13
working range
2-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
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General description
Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
Application
Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Larger bead size for large scale group separations where high flow rates and Low operating pressures are required.
- Classic gel filtration medium.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
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