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Merck
CN

GE17-0043-02

葡聚糖凝胶 ® G-50

Medium, Cytiva, pack of 500 g

别名:

SEC resin, buffer exchange resin, chromatography resin, cross-linked dextran, cytiva 17-0043-02, desalting matrix, filtration gel, size-exclusion resin

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化学文摘社编号:
9004-54-0
UNSPSC Code:
41115722
NACRES:
NA.56
MDL number:
MFCD23701222.obs1210407

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产品名称

葡聚糖凝胶 ® G-50, Medium, Cytiva, pack of 500 g

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

form

resin

crosslinking

cross-linked

mol wt

protein Mr 3-20 kDa (fractionation range)
protein Mr 30 kDa (exclusion limit)
Mp 0.5-10 kDa (dextrans fractionation range)

feature

autoclavable In wet form (pH 7.0) at 120°C for 30 min

packaging

pack of 500 g

manufacturer/tradename

Cytiva

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

matrix active group

dextran

swelling

1 g swells to 14-15 mL

particle size

103-320 μm (wet)
50-150 μm (dry)

cleaning in place

2-13

working range

2-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

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相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  • Classic gel filtration medium.

General description

Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Legal Information

Sephadex is a registered trademark of Cytiva

存储类别

13 - Non Combustible Solids

法规信息

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实验方案

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

使用Sephadex®进行分离

本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。

相关内容

Separation Options with Sephadex®

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

Sephadex® and Darcy’s Law

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

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