feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0060-01
matrix
cross-linked dextran
particle size
100-310 μm (wet), 40-120 μm (dry)
working range
2-10
General description
Sephadex® G-100 is useful for desalting and buffer exchange of very large biomolecules.
Application
Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Classic gel filtration medium.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
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实验方案
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Column Packing and Preparation for Size Exclusion Chromatography
本页介绍如何使用Cytiva的sephacryl色谱柱进行分离，该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。
相关内容
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
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