产品名称
Sephadex® LH-20, Cytiva 17-0090-02, pack of 500 g
form
resin
crosslinking
cross-linked
mol wt
protein Mr 4-5 kDa (fractionation range)
feature
autoclavable 20 min at 121°C
packaging
pack of 500 g
manufacturer/tradename
Cytiva 17-0090-02
parameter
60 cm/hr flow rate (recommended)
700 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
hydroxypropylated, cross-linked dextran
matrix active group
dextran
particle size
103 μm (in methanol)
70 μm (dry)
cleaning in place
2-13
working range
2-13
separation technique
size exclusion (SEC)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Features and Benefits
- Designed for use in polar organic solvents and aqueous solvent mixtures
- Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
- Useful for both analytical and industrial scales for the preparation of closely related molecular species
- Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
- Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
- Excellent batch-to-batch reproducibility
- Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
General description
Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.
Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.
Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility.
Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.
Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 25 °C ()
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Rajini Nagaraj et al.
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实验方案
Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.
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