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GE17-0404-01

HiTrap® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

别名:

Protein purification column

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NACRES:
NA.56
UNSPSC Code:
23151816
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form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0404-01

storage condition

(20% Ethanol)

parameter

1 mL/min flow rate, 4 mL/min max. flow rate, 5 bar max. pressure

technique(s)

liquid chromatography (LC): suitable

I.D. × L

7 mm × 25 mm

bed volume

1 mL

size

matrix

cross-linked agarose, spherical

matrix active group

Protein G

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

25 mg binding capacity (human IgG / mL resin)

separation technique

affinity

storage temp.

2-8°C

General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Warning

hcodes

H226

存储类别

3 - Flammable liquids

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实验方案

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

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Lin-Ping Wu et al.
Nature communications, 12(1), 4858-4858 (2021-08-13)
Complement is an enzymatic humoral pattern-recognition defence system of the body. Non-specific deposition of blood biomolecules on nanomedicines triggers complement activation through the alternative pathway, but complement-triggering mechanisms of nanomaterials with dimensions comparable to or smaller than many globular blood

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货号GTIN
GE17-0404-0104061837620096