form
prepacked column
crosslinking
cross-linked
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 17-0404-03
storage condition
(20% Ethanol)
parameter
1 mL/min flow rate, 4 mL/min max. flow rate, 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
I.D. × L
7 mm × 25 mm
bed volume
1 mL
size
matrix
(cross-linked agarose, spherical)
matrix active group
Protein G
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
25 mg binding capacity (human IgG / mL resin)
separation technique
affinity
storage temp.
2-8°C
General description
HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
- High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
- 34 μm bead size for high performance, high-resolution purifications.
- Binds broad range of different species and subclasses of IgG.
- Simple and proven method giving reproducible results.
- Designed for use with syringe, peristalic pump, or chromatography system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids