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Merck
CN

GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

别名:

HiTrap Protein G, Protein G affinity column

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NACRES:
NA.56
UNSPSC Code:
23151816

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产品名称

HiTrap® Protein G High Performance, Cytiva 17-0404-03, pack of 2 × 1 mL

form

prepacked column

crosslinking

cross-linked

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-0404-03

storage condition

(20% Ethanol)

parameter

1 mL/min flow rate
4 mL/min max. flow rate
5 bar max. pressure

technique(s)

liquid chromatography (LC): suitable

I.D. × L

7 mm × 25 mm

bed volume

1 mL

size

matrix

(cross-linked agarose, spherical)

matrix active group

Protein G

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

25 mg binding capacity (human IgG / mL resin)

separation technique

affinity

storage temp.

2-8°C

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

商品

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Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

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实验方案

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

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