表单
prepacked column
交联
cross-linked
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-0405-03
储存条件
(20% Ethanol)
参数
20 mL/min max. flow rate
5 bar max. pressure
5 mL/min flow rate
内径 × 长度
16 mm × 25 mm
柱床体积
5 mL
尺寸
基质
cross-linked agarose (spherical)
基质活性基团
protein G
平均零件尺寸
34 μm
cleaning
2-9
工作范围
3-9
容量
25 mg binding capacity (IgG / mL resin)
分离技术
affinity
储存温度
2-8°C
一般描述
特点和优势
- High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
- 34 μm bead size for high performance, high-resolution purifications.
- Binds broad range of different species and subclasses of IgG.
- Simple and proven method giving reproducible results.
- Designed for use with syringe, peristalic pump, or chromatography system.
制备说明
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
实验方案
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
General instructions for affinity purification using HiTrap columns from Cytiva.
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