ligand
iminodiacetic acid
包装
pack of 5 × 1 mL
制造商/商品名称
Cytiva 17-0408-01
参数
<4 mL/min flow rate
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-14
工作范围
3-13
一般描述
HiTrap® Chelating HP is prepacked with Chelating Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
应用
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System
特点和优势
- For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying Proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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