产品名称
HiTrap® Chelating High Performance, Cytiva 17-0409-01, pack of 1 × 5 mL
cleaning
2-14
working range
3-13
ligand
iminodiacetic acid
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-0409-01
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
Analysis Note
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying Proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System
General description
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
实验方案
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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