form
resin
crosslinking
cross-linked
packaging
pack of 300 mL
manufacturer/tradename
Cytiva 17-0510-01
storage condition
(20% ethanol)
technique(s)
liquid chromatography (LC): suitable
matrix
6% cross-linked agarose
matrix active group
-N+(CH3)3
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
2-12
capacity
0.18-0.24 mmol Cl-/mL ion exchange capacity, ~42 mg binding capacity (BSA / mL resin)
separation technique
strong anion exchange
shipped in
ambient
storage temp.
2-30°C
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General description
Q Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose™ Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose™ Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, Q Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Well-proven strong anion exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
wgk
WGK 1
法规信息
商品
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
实验方案
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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