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Merck
CN

GE17-0510-10

Q Sepharose™ Fast Flow

Cytiva 17-0510-10, pack of 25 mL

别名:

Q Sepharose Fast Flow

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UNSPSC代码:
41106500
NACRES:
NA.56

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表单

resin

交联

cross-linked

包装

pack of 25 mL

制造商/商品名称

Cytiva 17-0510-10

储存条件

(20% ethanol)

技术

liquid chromatography (LC): suitable

基质

6% cross-linked agarose

基质活性基团

-N+ (CH3 ) 3

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-14

工作范围

2-12

容量

0.18-0.24 mmol Cl-/mL ion exchange capacity
 ~42 mg binding capacity (BSA / mL resin)

分离技术

strong anion exchange

运输

ambient

储存温度

2-30°C

相关类别

一般描述

Q Sepharose™ Fast FLow is a strong anion exchanger based on the well established Sepharose™ Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

应用

  • Well-proven strong anion exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

特点和优势

Q Sepharose™ Fast FLow is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose™ Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose™ Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, Q Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

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Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed

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Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

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实验方案

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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