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Merck
CN

GE17-0510-10

Q Sepharose™ Fast Flow

Cytiva 17-0510-10, pack of 25 mL

别名:

Q Sepharose Fast Flow

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NACRES:
NA.56
UNSPSC Code:
41106500

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form

resin

crosslinking

cross-linked

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0510-10

storage condition

(20% ethanol)

technique(s)

liquid chromatography (LC): suitable

matrix

6% cross-linked agarose

matrix active group

-N+ (CH3 ) 3

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

2-12

capacity

0.18-0.24 mmol Cl-/mL ion exchange capacity, ~42 mg binding capacity (BSA / mL resin)

separation technique

strong anion exchange

shipped in

ambient

storage temp.

2-30°C

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General description

Q Sepharose™ Fast FLow is a strong anion exchanger based on the well established Sepharose™ Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Application

  • Well-proven strong anion exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Features and Benefits

Q Sepharose™ Fast FLow is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose™ Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose™ Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, Q Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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Pär Comstedt et al.
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There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed

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实验方案

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This page covers the use of Sepharose Fast Flow for purification of proteins.

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This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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