ligand
matrix surface
feature
autoclavable 20 min at 121°C
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0727-20
matrix
polystyrene/divinylbenzene
avg. part. size
15 μm (monosized)
cleaning in place
1-14
working range
1-12
capacity
~10 mg binding capacity
suitability
suitable for bioprocess medium
General description
Application
High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.
A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.
SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
- Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
- High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
- BioProcess medium supported for industrial applications.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.
This page discusses column packing and preparation techniques for reverse phase chromatography.
实验方案
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
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