form
resin
crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0886-02
storage condition
(20% Ethanol)
parameter
130 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
6% cross-linked agarose
matrix active group
recombinant GammaBind G type 3 lacking albumin-binding region
particle size
~90 μm
cleaning in place
2-9
operating pH
3-9
capacity
>20 mg binding capacity (human IgG/ml)
separation technique
affinity
shipped in
wet ice
storage temp.
2-8°C
General description
GammaBind Plus Sepharose™ is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to GammaBind Sepharose.
GammaBind Plus Sepharose™ is GammaBind G, Type 3, covalently immobilized to Sepharose™ CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose™ may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.
GammaBind Plus Sepharose™ is GammaBind G, Type 3, covalently immobilized to Sepharose™ CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose™ may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
- Recombinant Protein G type 3 coupled to Sepharose™ CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale.
- Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
- Yields up to 18 mg/mL mouse IgG.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids