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Merck
CN

GE17-0965-05

Phenyl Sepharose™ 6 Fast Flow / Low Sub

Cytiva 17-0965-05, pack of 200 mL

别名:

HIC purification matrix, aromatic HIC resin, chromatography resin, column packing, hydrophobic interaction chromatography polymer

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NACRES:
NA.56
UNSPSC Code:
41106500

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ligand

phenyl

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-0965-05

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

3-13

suitability

suitable for bioprocess medium

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General description

Phenyl Sepharose™ 6 Fast FLow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.

Application

Phenyl Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Standard aromatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring Low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

实验方案

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Selectivity and the Properties of a HIC Medium

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

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