ligand
human IgG
包装
pack of 10 mL
制造商/商品名称
Cytiva 17-0969-01
储存条件
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
基质
highly cross-linked 6% agarose
平均直径
90 μm
cleaning in place
3-12
工作范围
3-10
容量
~3 mg binding capacity(Protein A)
储存温度
2-8°C
相关类别
一般描述
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
特点和优势
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
制备说明
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
实验方案
This page shows how to purify protein A-tagged proteins using Cytiva products.
This page shows how to prepare samples for purification with affinity chromatography.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
相关内容
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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