ligand
butyl-S
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-0978-02
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
General description
Butyl-S Sepharose™ 6 Fast FLow is the least hydrophobic medium in the Sepharose™ Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.
Application
Butyl-S Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose™ 4 Fast FLow and other HIC Sepharose™ Fast FLow products.
This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)
Butyl-S Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)
Butyl-S Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
- The least hydrophobic medium in the well proven Sepharose™ Fast FLow HIC range
- Purifies recombinant Hepatitis B virus surface antigen from CHO cells
- Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
此项目有
商品
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
This page discusses column packing and preparation techniques for reverse phase chromatography.
实验方案
This page discusses various aspects of sample preparation for chromatographic purification.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持