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Merck
CN

GE17-0978-02

Butyl-s Sepharose 6 Fast Flow

Cytiva 17-0978-02, pack of 200 mL

别名:

HIC purification matrix, chromatography resin, column packing, hydrophobic interaction chromatography polymer, weak hydrophobic resin

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UNSPSC代码:
41106500
NACRES:
NA.56
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ligand

butyl-S

包装

pack of 200 mL

制造商/商品名称

Cytiva 17-0978-02

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-14

工作范围

3-13

适用性

suitable for bioprocess medium

相关类别

一般描述

Butyl-S Sepharose 6 Fast FLow is the least hydrophobic medium in the Sepharose Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.

应用

Butyl-S Sepharose 6 Fast FLow is part of the Sepharose Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose 4 Fast FLow and other HIC Sepharose Fast FLow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
  • The least hydrophobic medium in the well proven Sepharose Fast FLow HIC range
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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商品

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

This page discusses column packing and preparation techniques for reverse phase chromatography.

实验方案

This page discusses various aspects of sample preparation for chromatographic purification.

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