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Merck
CN

GE17-0980-01

Butyl Sepharose™ 4 Fast Flow

Cytiva 17-0980-01, pack of 200 mL

别名:

Butyl Sepharose, Fast Flow Sepharose

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NACRES:
NA.56
UNSPSC Code:
41106500

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ligand

butyl

packaging

pack of 200 mL

manufacturer/tradename

Cytiva 17-0980-01

matrix

4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

3-13

suitability

suitable for bioprocess medium

相关类别

General description

Butyl Sepharose™ 4 Fast FLow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins.

Application

Butyl Sepharose™ 4 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Optimized for the separation of larger Proteins in capture and intermediate purification steps
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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分析证书(COA)

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商品

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

实验方案

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Selectivity and the Properties of a HIC Medium

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

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