ligand
recombinant protein G lacking albumin-binding region
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva 17-1128-01
storage condition
(20% Ethanol)
parameter
<4 mL/min flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)
storage temp.
2-8°C
General description
MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Application
MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose™ High Performance.
Features and Benefits
- Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
- Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
- MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Still not finding the right product?
Explore all of our products under MAbtrap® Kit
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable