ligand
recombinant protein G lacking albumin-binding region
包装
pkg of 1 kit
制造商/商品名称
Cytiva 17-1128-01
储存条件
(20% Ethanol)
参数
<4 mL/min flow rate
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-9
工作范围
3-9
容量
>1 mg binding capacity (lgG/column)
储存温度
2-8°C
一般描述
MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
应用
MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose™ High Performance.
特点和优势
- Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
- Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
- MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
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