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Merck
CN

GE17-1128-01

MAbtrap® Kit

Cytiva 17-1128-01, pkg of 1 kit

别名:

IgG purification kit, affinity chromatography workflow, chromatography kit, protein G capture system

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NACRES:
NA.56
UNSPSC Code:
13111000

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ligand

recombinant protein G lacking albumin-binding region

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva 17-1128-01

storage condition

(20% Ethanol)

parameter

<4 mL/min flow rate, 42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

>1 mg binding capacity (lgG/column)

storage temp.

2-8°C

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General description

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose™ High Performance.

Features and Benefits

  • Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
  • Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
  • MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva

pictograms

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GHS07

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Warning

存储类别

3 - Flammable liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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商品

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

实验方案

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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