产品名称
HiTrap® SP High Performance, Cytiva 17-1152-01, pack of 5 × 5 mL
ligand
sulphopropyl
description
Ion Exchanger Type (value)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1152-01
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
24-44 μm
avg. part. size
34 μm
cleaning
3-14
working range
4-13
suitability
suitable for bioprocess medium
Analysis Note
Features and Benefits
- 34 μm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
- Strong sulfopropyl (SP) cation exchanger.
General description
The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
100.4 °F - closed cup
flash_point_c
38 °C - closed cup
法规信息
商品
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
实验方案
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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