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GE17-1152-01

HiTrap^® SP High Performance

Name: HiTrap<SUP>®</SUP> SP High Performance
Brand: SIGMA

Cytiva 17-1152-01, pack of 5 × 5 mL

别名:

HiTrap SP column

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关于此项目

NACRES:

NA.56

UNSPSC Code:

23151816

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属性

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-1152-01

parameter

<20 mL/min flow rate, 42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

说明

General description

HiTrap^® SP HP are prepacked, ready-to-use SP Sepharose^™ High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose^™ High Performance is a strong cation exchange medium and Q Sepharose^™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

Features and Benefits

34 μm bead size for high-performance, high-resolution purifications.
Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
Strong sulfopropyl (SP) cation exchanger.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)

Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

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pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 °F - closed cup

flash_point_c

38 °C - closed cup

法规信息

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分析证书(COA)

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实验方案

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

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全球贸易项目编号

货号	GTIN
GE17-1152-01	04061837621130