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Merck
CN

GE17-1177-01

Resource™ Q

Cytiva 17-1177-01, pack of 1 mL

别名:

Resource Q Column

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UNSPSC代码:
47101511
NACRES:
NA.56

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表单

prepacked column

交联

cross-linked

包装

pack of 1 mL

制造商/商品名称

Cytiva 17-1177-01

储存条件

(20% ethanol)

参数

1-10 mL/min flow rate
1.5 Mpa max. pressure
10 mL/min max. flow rate

技术

liquid chromatography (LC): suitable

柱床体积

1 mL

色谱柱内径 × 长度

6.4 mm × 30 mm

基质

polystyrene/divinylbenzene

基质活性基团

CH2–O–CH2–CHOH–CH2–O–CH2– CHOH–CH2–N+ (CH3)3

平均零件尺寸

15 μm

cleaning

1-14

工作范围

2-12

容量

~45 mg binding capacity (BSA / mL resin)

分离技术

anion exchange

运输

ambient

储存温度

2-30°C

相关类别

一般描述

RESOURCE™ Q columns are prepacked with SOURCE™ 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

特点和优势

  • SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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实验方案

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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