form
prepacked column
crosslinking
cross-linked
packaging
pack of 6 mL
manufacturer/tradename
Cytiva 17-1179-01
storage condition
(20% Ethanol )
parameter
0.6 Mpa max. pressure, 1-60 mL/min flow rate (recommended), 60 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
6 mL
column I.D. × L
16 mm × 30 mm
matrix
polystyrene/divinylbenzene
matrix active group
CH2–O–CH2–CHOH–CH2–O–CH2– CHOH–CH2–N+ (CH3)3
avg. part. size
15 μm
cleaning
1-14
working range
2-12
capacity
~ 45 mg BSA/mL resin binding capacity
separation technique
anion exchange
shipped in
ambient
storage temp.
2-30°C
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General description
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Application
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Features and Benefits
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
实验方案
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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