form
prepacked column
crosslinking
cross-linked
packaging
pack of 6 mL
manufacturer/tradename
Cytiva 17-1179-01
storage condition
(20% Ethanol )
parameter
0.6 Mpa max. pressure, 1-60 mL/min flow rate (recommended), 60 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
6 mL
column I.D. × L
16 mm × 30 mm
matrix
polystyrene/divinylbenzene
matrix active group
CH2–O–CH2–CHOH–CH2–O–CH2– CHOH–CH2–N+ (CH3)3
avg. part. size
15 μm
cleaning
1-14
working range
2-12
capacity
~ 45 mg BSA/mL resin binding capacity
separation technique
anion exchange
shipped in
ambient
storage temp.
2-30°C
General description
RESOURCE™ Q columns are prepacked with SOURCE™ 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Application
Automated pilot scale purification of synthetic oligonucleotides; AKTAexplorer Summary
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Features and Benefits
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
Still not finding the right product?
Explore all of our products under Resource™ Q
signalword
Warning
hcodes
存储类别
3 - Flammable liquids