表单
prepacked column
交联
cross-linked
包装
pack of 6 mL
制造商/商品名称
Cytiva 17-1179-01
储存条件
(20% Ethanol )
参数
0.6 Mpa max. pressure
1-60 mL/min flow rate (recommended)
60 mL/min max. flow rate
技术
liquid chromatography (LC): suitable
柱床体积
6 mL
色谱柱内径 × 长度
16 mm × 30 mm
基质
polystyrene/divinylbenzene
基质活性基团
CH2–O–CH2–CHOH–CH2–O–CH2– CHOH–CH2–N+ (CH3)3
平均零件尺寸
15 μm
cleaning
1-14
工作范围
2-12
容量
~ 45 mg BSA/mL resin binding capacity
分离技术
anion exchange
运输
ambient
储存温度
2-30°C
一般描述
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
应用
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
特点和优势
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
制备说明
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to perform a purification of His-tagged membrane proteins.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
实验方案
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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