material
polypropylene (PP)
mol wt
5000-250000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-1195-01
parameter
<13 mL sample volume, <2.6 mL/min flow rate, 0.15 Mpa (22 psi)
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric
matrix active group
dextran-acrylamide phase
avg. part. size
50 μm
cleaning
2-13
working range
3-11
separation technique
size exclusion (SEC)
General description
HiPrep™ 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.
Application
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume
Features and Benefits
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
- Gel filtration columns for low to medium pressure AKTA systems.
- Well-established in industrial production.
- The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
Preparation Note
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
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实验方案
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
This page shows how to refold proteins using gel filtration and products from Cytiva.
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
相关内容
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.