ligand
phenyl
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-1355-01
parameter
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium
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General description
Features and Benefits
- Provide convenient and fast small-scale Protein purifications.
- Phenyl Sepharose™ 6 Fast FLow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring Low to medium hydrophobicity. Two levels of substitution on Phenyl Sepahrose 6 Fast FLow help find optimal selectivity and binding capacity.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
- High flow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
实验方案
This page discusses various aspects of sample preparation for chromatographic purification.
This page shows how to prepare samples for purification with affinity chromatography.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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