form
resin
crosslinking
cross-linked
manufacturer/tradename
Cytiva 17371201
storage condition
(20% ethanol)
parameter
600 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
Highly cross-linked spherical agarose
matrix active group
Nickel
particle size
~90 μm
cleaning in place
2-14
operating pH
2-12
capacity
≥10 mg binding capacity (histidine-tagged protein)
separation technique
affinity
storage temp.
2-30°C
General description
)Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6-tagged pure protein (Mr 43 000) in EX-CELL 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.
Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
- HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
- His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.
Features and Benefits
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
- Retained binding capacity of up to 10 mg/mL resin.
- Simplified sample handling – fewer steps needed compared with conventional workflow.
- Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.
Preparation Note
Store at 4 to 30 °C (20% ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids