form
resin
crosslinking
cross-linked
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-3712-02
storage condition
(20% ethanol)
parameter
600 cm/hr max. flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
Highly cross-linked spherical agarose
matrix active group
Nickel
particle size
~90 μm
cleaning in place
2-14
operating pH
2-12
capacity
≥10 mg binding capacity (histidine-tagged protein)
separation technique
affinity
storage temp.
2-30°C
General description
Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.
Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6- tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.
Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
- HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
- His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.
Features and Benefits
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
- Retained binding capacity of up to 10 mg/mL resin.
- Simplified sample handling – fewer steps needed compared with conventional workflow.
- Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
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存储类别
3 - Flammable liquids