ligand
diethylaminoethyl
描述
Ion Exchanger Type (value)
包装
pack of 5 × 1 mL
制造商/商品名称
Cytiva 17-5055-01
参数
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14
工作范围
2-12
适用性
suitable for bioprocess medium
相关类别
一般描述
HiTrap® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
应用
Q, SP, DEAE, and CM Sepharose™ Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
特点和优势
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High fLow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
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