ligand
recombinant protein A (E. coli)
包装
pack of 2 × 1 mL
制造商/商品名称
Cytiva 17-5079-02
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
<4 mL/min flow rate
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
4% cross-linked agarose
粒径
60-165 μm
平均直径
90 μm (d50v)
cleaning
2-11
工作范围
3-10
容量
~50 mg binding capacity (human lgG/ml medium)
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
应用
The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
特点和优势
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established Protein A-based medium used in many approved MAb processes.
- Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA® design.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持