ligand
2-mercaptopyridine
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-5111-01
storage condition
(20% Ethanol)
parameter
<20 mL/min flow rate, 42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
3-11
storage temp.
2-30°C
General description
HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose™ High Performance for fast and easy purification of IgY from egg yolk.
Application
HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
Features and Benefits
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
- Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose™ High Performance.
- Recovery and purity of IgY are excellent.
- Can be operated with a liquid chromatography system such as ÄKTA® design.
- The top and bottom frits are manufactured from porous polyethylene
- Connectors for usage with different chromatography systems and other equipment are included
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids