ligand
p-aminobenzamidine
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5123-10
storage condition
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning in place
1-9
working range
2-8
storage temp.
2-8°C
General description
Application
Features and Benefits
- Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
- p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
- For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
实验方案
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
This page shows how to prepare samples for purification with affinity chromatography.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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