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GE17-5132-01

Glutathione Sepharose™ 4 Fast Flow

Cytiva 17-5132-01, pack of 25 mL

别名:

FPLC column packing, GST affinity matrix, affinity resin, glutathione s-transferase affinity polymer, stationary phase

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NACRES:
NA.56
UNSPSC Code:
41106500
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form

resin

crosslinking

cross-linked

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5132-01

storage condition

(20% Ethanol)

parameter

100 cm/hr max. flow rate (sample loading), 100-300 cm/hr flow rate (wash and elution)

technique(s)

liquid chromatography (LC): suitable

matrix

highly cross-linked 4% agarose

matrix active group

Glutathione

particle size

~90 μm

cleaning

3-12

operating pH

3-12

capacity

>10 mg/mL binding capacity

separation technique

affinity

storage temp.

2-30°C

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4 Fast Flow, 25 mL
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

Features and Benefits

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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分析证书(COA)

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实验方案

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

商品

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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Luis J García-Rodríguez et al.
Current biology : CB, 29(9), 1536-1544 (2019-04-23)
For proper chromosome segregation in mitosis, sister kinetochores must interact with microtubules from opposite spindle poles (chromosome bi-orientation) [1, 2]. To promote bi-orientation, Aurora B kinase disrupts aberrant kinetochore-microtubule interactions [3-6]. It has long been debated how Aurora B halts this

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货号GTIN
GE17-5132-0104061837621765