form
resin
crosslinking
cross-linked
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-5132-02
storage condition
(20% ethanol)
parameter
100 cm/hr max. flow rate (sample loading), 100-300 cm/hr flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 4% agarose
matrix active group
Glutathione
particle size
~90 μm
cleaning
3-12
operating pH
3-12
capacity
>10 mg/mL binding capacity
separation technique
affinity
storage temp.
2-30°C
General description
Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
Features and Benefits
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids